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Then I run a NJ analysis to see what is going on with the dataset.

Guide to editing sequences with Chromas and BioEdit I copy the sequence titles to the clipboard Edit, Copy sequence titles. I manually align them and check for obvious missing bases and either correct them or add a gap to preserve the alignment. Click on the view menu for the original unedited fileand check Reverse Complement. Select the value you wish to change, hit the value on the keyboard and that will reset it. Overwrite the sequence title onto the next title shifting up, when the title is being edited. MEGA also has an alignment editor, but I’ve not really used it very much. BioEdit can also edit chromatograms, but I find Chromas to be nicer. BioEdit is a mouse-driven, easy-to-use sequence alignment editor and sequence analysis program designed and written by a graduate student. This is likely to be the final release of BioEdit. North Carolina State University, Department of Microbiology.